胰岛素样生长因子结合蛋白-2水平与肝癌关系的研究

Relationship between insulin-like growth factor binding protein-2 (IGFBP-2) and hepatocellular carcinoma

目的探讨血清胰岛素样生长因子结合蛋白-2(IGFBP-2)水平与肝细胞癌的关系及其在肝癌的诊断、治疗中潜在价值。方法采用酶联免疫吸附试验ELISA法测定肝细胞癌组(54例)血清IGFBP-2、IGFBP-3、AFP、IGF-1、IGF-2、GH水平与肝硬化组(20例)和健康对照组(32例)结果对照比较。结果肝细胞癌组血清IGFBP-2水平明显高于肝硬化组和健康对照组(t=4.63,P<0.05,t=3.73,P<0.01),肝硬化组和健康对照组比较差异无统计学意义。肝癌切除术后4周IGFBP-2水平明显下降,与术前相比差异有统计学意义(t=3.52,P<0.05)。肝细胞癌组GH、IGF-1、IGF-2、IGFBP-3水平与肝硬化组对比差异无统计学意义。相关性分析显示肝细胞癌组IGFBP-2与AFP水平呈正相关(r=0.51,P<0.05),与GH、IGF-1、IGF-2、IGFBP-3无相关性。结论肝细胞癌患者IGFBP-2水平的异常增高可能与肝癌细胞合成释放增加有关。与AFP结合使用血清IGFBP-2检测可成为肝癌筛查、监测的有效手段。

Objective To evaluate the role of insulin- like growth factor binding protein- 2 （IGFBP- 2） level in the hepatocellular carcinoma （HCC） and contribution to incidence of HCC and possibility in the diagnostic work - up of patients with HCC. Methods The basal serum level of IGFBP - 2/3 ,AFP,IGF - 1, IGF - 2 and GH in 54 patients with HCC confirmed histologically was compared with that in patients with hepatic cirrhosis and healthy controls. Results The level of IGFBP- 2 in the patients with HCC was higher than in the hepatic cirrhosis group and control group （ t = 4.63, P 〈 0.05 ; t = 3.73, P 〈 0.01 ）. Four weeks after the resection of HCC,the level of IGFBP- 2 was decreased （ t = 3.52, P 〈 0.05 ）. There was no significant difference in the serum levels of GH, IGF- 1/2 and IGFBP- 3 between HCC group and control group. In HCC,the serum level of IGFBP- 2 and AFP had a positive correlation （ r = 0.51, P 〈 0.05 ）. Conclusion Apart from AFP, IGFBP - 2 appears to be a suitable marker of the HCC,which can be applied in the diagnosis of patients with HCC.