异基因供体骨髓细胞胸腺内注射对肠移植大鼠的影响

Role of intrathymic injection of allogene bone marrow in rats following small intestine transplantation

目的:观察异基因骨髓注射在大鼠小肠移植中的免疫耐受作用。方法:实验于2006-09/2007-03在平凉市第二人民医院及兰州大学第二医院完成。①将大鼠分为空白对照组(Wistar)、同基因移植组(FK344/N)、异基因移植组(Wistar)、骨髓胸腺内注射组(Wistar),空白对照组18只,其余每组18只受体大鼠,供体为18只FK344/N大鼠。除空白对照组外,各组选用供受体大鼠进行全小肠异位移植,骨髓胸腺内注射组在行异基因小肠移植前7d取供体骨髓细胞行受体胸腺内注入,同基因移植组、异基因移植组大鼠不注射异基因骨髓细胞,只进行全小肠移植。②每组大鼠分别于术后3,5,7d观察排斥反应,于各时间点每次处死6只,检测血清可溶性白细胞介素2受体表达,并取出移植肠进行苏木精-伊红染色后观察组织学变化。结果:纳入受体大鼠54只及空白对照组大鼠18只均进入结果分析。①排斥反应:异基因移植组大鼠异位全小肠移植术后3,5,7d可出现典型的轻、中、重度排斥反应,而同基因组和骨髓胸腺内注射组中未出现排斥反应。②可溶性白细胞介素2受体水平:术后3d,同基因移植组、骨髓胸腺内注射组可溶性白细胞介素2受体表达水平高于空白对照组(P<0.01),而第5,7天与空白对照组差异不显著(P>0.05),异基因移植组受体大鼠各时间点血清可溶性白细胞介素2受体表达均高于同基因移植组(P<0.01)。结论:移植术前7d异基因供体骨髓在受体胸腺内注射能显著减少小肠移植后急性排斥反应的发生。血清可溶性白细胞介素2受体的检测可能作为小肠移植急性排斥反应的早期诊断敏感的免疫指标。

AIM： To investigate the immune tolerance of allogene bone marrow injection to the rat small intestine transplantation. METHODS： The experiment was carried out from September 2006 to March 2007 in Pingliang Second Hospital of Gansu Province and the Second Hospital of Lanzhou University. ①Rats were divided into blank control group （n =18 Wistar）, isogeneic transplantation group （FK344/N）, allogene transplantation group （Wistar）, and intrathymic injection group （Wistar）, 18 donator rats and 18 receptor FK344/N rats for later four groups. All the rats except the blank control group were selected to perform heterotopic graft to the whole small intestine. Seven days before allogene transplantation, donator bone marrow cells were injected into thymus of receptors （test group）. The isogene and allogene transplantation groups received only the whole small intestine, without injection. ③Six rats of each group were executed at 3, 5, 7 days postoperation to observe the reject reacion and detect the expression of serum soluble intedeukin-2 receptor （slL-2R）. The histological change of graft intertine was observed by hematoxylin-eosin staining. RESULTS： All the rats in blank control group and receptor ones were included in the result anlysis.①Reject reaction： At 3, 5 and 7days after dystopia whole small intestine transplantation in allogeneic rats, typical reject reactions appeared, but there was no reject reaction in isogenome and test group.②Level of serum slL-2R： 3 days after allotransplantation, serum slL-2R level of isogeneic transplantation group and test group was significantly higher than that of blank control group （P〈0.01）, but the difference was insignificant at the 5^th and 7^th days （P〉0.05）. The level of serum slL-2R in allogene transplantation group was higher than that of isogeneic transplantation group all the time （P〈0.01）. CONCLUSION： AIIogenic donator bone marrow intrathymic injecting into receptor 7 days before small intestine transplantation, can reduce the acute reject reaction after grafting. The level of serum slL-2R can be selected as a sensitive early diagnosis index of acute rejection after small intestine transplantation.