Iloprost液4℃保存的胎鼠皮层组织移植修复脑损伤的实验研究

The repair of injured brain by implanting embryonic cortical brain tissue preserved in lloprost solution at 4℃:experimental study

为探讨脑组织移植治疗脑损伤及供体组织的保存,作者在制造大鼠运动区皮层损伤的基础上,分别以新鲜及Iloprost液或生理盐水保存3小时的胎鼠皮层组织为供体,将其移植到损伤的大鼠中,观察移植后的神经行为变化及形态学改变。结果表明,移植各组大鼠的神经行为较损伤组明显改善,新鲜组织及Iloprost液保存组织移植基本恢复至伤前水平。Iloprost液保存组织移植的成活率明显高于生理盐水保存组织移植的成活率(P<0.05),而与新鲜组织相比相差不显著(P>0.05)。此外Iloprost液保存组织移植后的生长体积较新鲜组织移植明显增加(P<0.01)。以上结果提示,通过移植胎鼠皮层可有效地修复脑损伤,Iloprost液不仅可在4℃保持移植物的活性,还可促进其移植后的存活、生长。

We transplanted the fresh and Iloprost or saline preserved(3 hours) embryonic rat cortical brain tissue into the rats in which the motor cortex had been injured and compared the neurobehavioral and morphological changes. The neurobehavior of the transplanted groups was more significantly improved than that in the injured, untransplanted group. The neurobehavior of the animals of the fresh tissue group and Iloprost preserved group recovered to the preinjurylevel. The survival rate of the Iloprost preserved grafts was 90%, which was higher than that of the saline preserved grafts(P<0.05). In comparison with the fresh grafts, howerev, there was no significant difference(P>0.05). In addition, the growth volume of the Iloprost preserved grafts was more markedly increased than that of the fresh grafts(P<0.01). We conclude that injured brain may be repaired effectively by transplantation of fetal brain cortex. The Iloprost solution not only maintains of vitality the grafts, but also promotes its survival and growth in the host brain.