内毒素性肺损伤大鼠肺表面活性物质的变化

AN EXPERIMENTAL STUDY OF PULMONARY SURFACTANT CHANGES AFTER LUNG INJURY INDUCED BY ENDOTOXIN IN RATS

大鼠注射内毒素后１～７２ｈ６个时相点，肺组织主要病变为充血、瘀血、水肿，不同程度的肺萎陷；肺泡Ⅱ型上皮（ＰⅡ）肿胀、退变。肺组织匀浆表面活性物质（ＰＳ）含量及活性下降，肺泡隔面积密度相应增加，二者呈显著负相关（ｒ＝－０．９３９５，Ｐ＜０．０１）。于注射内毒素后１～４８ｈ改变最为明显。结果表明，内毒素可直接或间接致ＰⅡ及ＰＳ系统受损，进而促进肺水肿、肺萎陷的发生和呼吸窘迫综合征的形成。

The ultrastructural changes of lung,pulmonary homogenates sufactant (ST) and the area density of alveolar septa were investigated at 1,5,12,24,48 and 72h after lipopolysaccharide (LPS) injection.The most prominent changes were pulmonary tissue congestion,edema,collapse,type Ⅱ epithelial cells (PⅡ) swelling and degeneration.ST content and it's activity decieased while the area density of alveolar sepat increased.They had a significanly negtive correlation ( r =-0 9395, P <0 01).The above changes were more severe 1 to 48h after LPS injection.The results indicated that LPS may cause direct or indirect damage to the PⅡ and ST system and that it accelerate the development of pulmonary edema,collapse and respiratary distress syndrome.