急性白血病微量残留细胞定量分析及其临床意义

Quantitative analysis of minimal residual cell of acute leukemia and its clinical significance

为了研究残留白血病细胞数量变化的临床意义，应用竞争性聚合酶链反应（ＰＣＲ）技术定量检测急性淋巴细胞白血病患儿的ＴｃＲγ基因。结果：４９例患儿缓解后１～６４个月中共收集到１２６份骨髓标本，有３３例５１份标本检测到残留白血病细胞（０．００６％～３．３％），且残留白血病细胞数量呈逐年减少趋势。有６例患儿复发，其复发前残留白血病细胞的定量高于未复发组（复发组为０．５６％±０．４７％，未复发组为０．１７％±０．１１％，Ｐ＜０．０１）。提示：残留白血病细胞定量检测有助于指导治疗，判断预后。

To study the relationship between the quantity of residual leukemic cell and the clinical outcome in children with acute lymphoblastic leukemia (ALL), the authors detected quantitatively the TcRγ gene by using the competitive PCR technique. In 126 bone morrow samples collected from 49 ALL patients during the follow up period of 1～64 months after clinical remission, the 51 samples of 33 patients were discovered to have the residual leukemic cell of different amount (0 006%～3 3%), which appeared to have a tendency to diminish gradually year by year. Of the 49 patients, 6 had clinical relapse, in whom the amounts of residual leukemic cell before relapse were higher than that in non relapse patients (0 56%±0 47%, 0 17%±0 11%, P <0 01). These results suggest that quantitative analysis of minimal residual leukemic cell is helpful in guiding treatment, evalualing the prognosis and prediciting relapse.