摘要
通过实验室生态毒理实验,研究黑鲷(Sparus macrocephalus)暴露苯并(a)芘(BaP)后,鱼体胆汁中代谢产物3-羟基-苯并(a)芘(3-OHBaP)的剂量-时间-效应关系,结果显示:胆汁中3-OHBaP的浓度随暴露时间的延长呈现先上升然后在7d后开始逐渐降低的变化趋势;胆汁中3-OHBaP浓度随暴露浓度的升高而不断升高,呈现明显的剂量-效应关系.表明胆汁中的3-OHBaP反映了环境中母体BaP的浓度,是一个有效的污染监测生物标志物,3-OHBaP对于较短时间BaP暴露具有很好的指示作用.
In this paper, the dose- and time response of metabolites of benzo（a） pyrene（BaP） in marine fish （black porgy, Sparus macrocephalus） were studied to evaluate as a means of assessing exposure to PAHs. The fish were exposed to different concentrations of BaP （0. 5, 1.0, 2.0 and 5.0μg·l^-1 ） for 14d and then depurated 7d. The samples of fish bile were collected at 2h, 6h, 12h, 1d, 2d, 4d, 7d, 14d and 21d respectively. BaP metabolites were separated and quantified by reverse-phase HPLC with fluorescence detector. The results showed that 3-hydroxy-benzo（a） pyrene （3-OH BaP） was the most abundant metabolite presented in fish bile and occupied 75%-82% of all BaP metabolites. The biliary BaP increased with the exposure time and reached the peak at the 4^th day or 7^th day and then decreased. A significant dose-related increase of the metabolites concentrations in bile was observed at the exposure time of 2d, 4d and 7d （p 〈0. 05 ） . These results demonstrated that 3-OH BaP concentration in fish bile is a certainly sensitive biomarker to assess recent exposure to BaP.
出处
《环境化学》
CAS
CSCD
北大核心
2007年第4期487-490,共4页
Environmental Chemistry
基金
国家自然科学基金(40106012和40476048)资助项目
