携带HuIL-3基因的人肝癌特异性逆转录病毒载体的构建

Construction of retroviral vector carrying human IL-3 cDNA under control of human AFP enhancer core sequence and SV40 early region promoter

目的:构建携带人白细胞介素3（HuIL－3）cDNA的人肝癌特异性逆转录病毒载体。方法和结果：将HuIL－3cDNA克隆到逆转录病毒载体pMNSM的PL位点，构建SV40早期启动子驱动的载体pMNS－IL－3；从质粒pGEM．7Zf－AFPe中游离人甲胎蛋白（AFP）增强子核心序列，先克隆到pMNSM的PL位点上，再将HuIL－3cDNA片段克隆到PL位点上，构建出SV40早期启动子和AFP增强子驱动的人肝癌特异性表达载体PMNSA－IL－3。结论：该载体为肝癌特异性转基因治疗提供了一条途径。

To construct retroviral vector carrying human interleukin-3 c0mplementary DNA(HuIL-3 cDNA) under control of human a-fetoprotein gene enhancer core sequence and human SV4O pro-moter. Methods and Results: HuIL-3 cDNA was inserted into polylinker site of retroviral vector pMNSMto construct retr0viral vector pMNS-IL-3, in which the transcription of HuIL-3 cDNA was drived by SV40early region promoter. Human Q-fetoprotein gene enhancer core sequence was released from plasmidpGEM. 7Zf-AFPe and inserted into the polylinker site of pMNSM. Then human interleukin-3 cDNA wasinserted int0 p0lylinker site to construct retroviral vector pMNSA-IL-3, in which HuIL-3 cDNA transcrip-tion was drived by SV40 early region promoter and enhanced by human a-fetoprotein enhancer core se-quence- Conclusion: The vectors are of significance for hepatoma-specific gene therapy.