(131)~碘标记抗人AFP-R-LCA McAb对原发性肝癌的放射免疫显像

The clinical study of anti-human AFP variant McAb in radioimmuno detection for primary hepatocellular carcinoma

目的:探讨抗人小扁豆凝集素结合型甲胎蛋白异质体单克隆抗体（AFP－R－LCAMcAb）对原发性肝癌细胞的特异性亲和力及放射免疫显像。方法：应用(131)Ⅰ标记的AFP－R－LCAMcAb对11例AFP阳性肝癌、6例AFP阴性肝癌及4例肝炎后肝硬化患者进行放免显像，观察不同时间肝癌显像情况。结果：11例AFP阳性肝癌患者中有9例肿瘤区显像，而6例AFP阴性肝癌及4例肝炎后肝硬化患者无1例出现肝肿瘤区及肝内局部显像。且小剂量(131)I－AFP－R－LCAMcAb对人体肝功能及凝血酶原时间无明显影响（P＜0．05）。结论：AFP－R－LCAMcAb对人AFP阳性肝癌细胞有较强的特异性亲和力，有希望成为肝癌放免检测及治疗的理想载体。

To research into the special affinity of AFP-R-LCA monoclonal antibody (AFP-R-LCA McAb) to AFP positive primary hepatocellular carcinoma cells. Methods: AFP-R-LCA McAb waslabeled by 131I and 11 patients with positive AFP, 6 with negative AFP and 4 liver cirrhosis patients afterhepatitis B were investigated with radioimmuno-detection. Results: The 131I-AFP-R-LCA McAb was gath-ered in human hepatocellular carcinorna (HCC) of 9 AFP positive patients (9/11), but there was no posi-tive gathering in HCC of 6 AFP negative or 4 liver cirrhosis patients after hepatitis B and 131I-AFP-R-LCAMcAb at a small dose was no side effects for liver function test and PT test (P>O. O5). Conclusion:AFP-R-LCA McAb has a strong and special affinity to AFP positive cells and can be recognized as a carrier f0rradioimmunodetection and radioimmunotherapy.