1,25-二羟维生素D_3对其D_3受体mRNA表达的细胞特异性调节

Tissue-specific regulation of vitamin D_3 receptor mRNA expression by 1, 25-dihydroxyvitamin D_3

目的:以人原始巨核白血病细胞系（HIMeg）和人骨肉瘤细胞系（HOS－8603）为细胞模型，研究1，25一二羟维生素D3[1，25（OH）2D3]对维生素D3受体（VDR）mRNA表达的调节作用。方法：VDRmRNA的检测采用定量逆转录．聚合酶链反应（RT－PCR）。结果：HOS－8603细胞经1，25（OH）2D3处理0～24h后，VDRZRNA表达水平无明显变化；但是1，25（OH）2D3却可以使HIMeg细胞VDRmRNA水平明显降低，且具有时间依赖性，24h后达到最低水平，仅为对照组的15％。结论：1，25（OH）2D3对VDRmRNA表达的调节作用具有组织细胞特异性。

To explore the potential regulatory effects of 1, 25-dihydroxyvitamin D, L1' 25 (0H),D,j on the expression of vitamln D, receptor (VDR ) mRNA in human megakaryoblastic leukemia(HIMeg) and human osteosarcoma (HOS-86O3) cell lines. Methods: VDR mRNA expression was deter-mined by the utilization of our recently established quantitative reverse-transcripti0n p0lymerase chain re-acti0n (RT^PCR) method. Results: lt was found that VDR mRNA expression in HOS-86O3 cells was notchanged significantly after treatment with 1, 25 (OH),D, for O~ 24 h- On the other hand, treatment 0fHIMeg ce1ls with 1, 25 (OH),D, cou1d significantly induce a d0wn-regulati0n of VDR mRNA expressi0n ina time-dependent manner, reaching a level of about only 15% of control after 24 h treatment. C0ncIusi0n:The regulation of VDR mRNA expressi0n by 1,25 (OH), D, is of tissue-specific fashi0n.