摘要
目的:观察增殖细胞核抗原(PCNA)及核仁组成区嗜银蛋白(AgNOR)在舌良恶性肿瘤中的表达及数量变化。方法;运用免疫组化ABC法及核仁组成区银染色技术检测36例人舌鳞状细胞癌、14例舌乳头状瘤中PCNA阳性指数和AgNOR值的变化。结果:PCNA阳性指数分别为乳头状瘤7.15±1.32,舌鳞癌24.28±2.47,鳞癌Ⅰ级19.45±15.88,鳞癌Ⅱ级22.5±18.79,鳞癌Ⅲ级65.3±17.39,舌鳞癌组与舌乳头状瘤组间有非常显著差异(P<0.01)、鳞癌Ⅲ级与鳞癌Ⅰ级、鳞癌Ⅱ级间有显著差异(P<0.05)及非常显著差异(P<0.01)。AgNOR值分别为舌乳头状瘤2.16±0.33,舌鳞癌4.76±1.59,两者有非常显著差异(P<0.01)。鳞癌Ⅰ级、Ⅱ级、Ⅲ级AgNOR值分别为4.61±1.76,4.75±0.94,4.93±1.70,3组间无显著差异。结论:PCNA及AgNOR阳性表达与肿瘤细胞的增殖活性有关,可用以辅助鉴别诊断良恶性病变。前者还可作为评估肿瘤恶性程度的一项参考指标。
To clarify the difference between benign and malignant tumours of tongue on the PC-NA expression and AgNOR c0unts. Methods: Immunohistochemical and AgNOR stainings were carriedout to detect the PCNA expressi0n and to count AgNOR particles in 14 cases of papillomas and 36 cases ofsquamous cell carcinomas of tongue. Results: The positive index(PI) of PCNA was 7. 15 ± 1. 32 in papillo-mas and 24. 28± 2. 47 in squamous cell carcinomas, and there was a significant difference between them. Asignificant difference between low 19. 45± 15. 88, medium 22. 5 ±18. 79 and high grade carcin0mas 65. 3 ±17. 39 was also observed. The AgNOR counts were higher in squamous cell carcinomas 4. 76 ±1. 59 than inpapillomas 2. 16 ± 0. 33. There was no difference of AgNOR counts in high, medium and low grade ofsquamous cell carcinomas. Conclusion:The PCNA expression and AgNOR counts may be related to activi-ty of cell proliferation and be helpful for differentiating benign from malignant tumours of tongue. In addi-tion, the former may be helpful for grading malignancy.
出处
《第二军医大学学报》
CAS
CSCD
北大核心
1997年第1期77-79,共3页
Academic Journal of Second Military Medical University
关键词
舌肿瘤
乳头状瘤
鳞状细胞癌
PCNA
AGNOR染色
tongue neoplasms
papilloma
squamous cell carcinoma
proliferating cell nuclear antigen
AgNOR