松乳菇深层培养胞外聚合物产生的营养条件的研究

Study on Nutrient Conditions for the Mycelial Growth and Exo-biopolymer Production by Submerged Culture of Lactarius deliciosus

研究了松乳菇深层培养所需的碳、氮源及其交互作用。在供试的多种碳、氮源中,最适合菌丝体生长和胞外生物聚合物产生的碳源是蔗糖和葡萄糖;最适合菌丝体生长的氮源是牛肉膏和蛋白胨,最适合胞外生物聚合物产生的氮源是黄豆粉,松乳菇不能利用尿素和亚硝酸盐。碳、氮源交互试验结果表明:松乳菇菌丝体生长和胞外生物聚合物产生的优良培养基为培养基1:蔗糖1.5%、可溶性淀粉1.5%、牛肉膏0.25%、黄豆粉1%、硝酸钠0.1%;菌丝体和胞外生物聚合物的生物量分别为0.923g/100mL和80.1mg/100mL;培养基2:蔗糖1.5%、可溶性淀粉2%、牛肉膏0.1%、黄豆粉0.75%、硝酸钠0.3%;菌丝体和胞外生物聚合物的生物量分别为0.927g/100mL和83.2mg/100mL。

This paper presents a research on carbon and nitrogen resources by submerged culture of Lactarius deliciosus. The result show that the sucrose and the glucose are the optimal carbon resources for mycelial growth and exo - biopolymer production; the optimal ni- trogen resources for mycelial growth are the beef extract and pepton, the optimal nutrogen resources for exo - biopolymer production are soy powder and beef extract, urea and nitrite can not be utilized. The orthogonal test results show that the optimal medium for mycelial growth and exo - biopolymer production are media 1 ： sucrose 1.5%, soluble starch 1.5%, beef extract 0. 25%, soy powder 1%, NaNO3 0. 1%, the mycelial and exo - biopolymer output in this media are 0. 932g/100mL and 80. 1 mg/100mL seperatively; and media 2： sucrose 1.5%, soluble starch 2%, beef extract 0. 1%, soy powder 0. 75%, NaNO3 0.3%, the mycelial and exo - biopolymer output in this media are 0. 927g/100mL and 83.2mg/100mL seperatively.