婴儿巨细胞病毒肝炎IFN-γ+874单核苷酸多态性研究

Interferon-γ+874 gene polymorphism in infant cytomegalovirus hepatitis

目的探讨IFN-γ+874单核苷酸多态性与婴儿巨细胞病毒肝炎的关系。方法对87例婴儿巨细胞病毒肝炎和同期入院的89例非巨细胞病毒感染患儿,应用ABI Prism 7700高通量荧光PCR系统进行IFN-γ+874单核苷酸多态性测定。结果婴儿巨细胞病毒肝炎患儿中64例为AA型,20例为AT型,3例为TT型;对照组中45例为AA型,26例为AT型,18例为TT型,经卡方检验两组差异有统计学意义(P<0.01)。婴儿巨细胞病毒肝炎患儿治疗恢复顺利者65例,43例为AA型,19例为AT型,3例为TT型;恢复不顺利者22例,21例为AA型,1例为AT型,经卡方检验两组差异有统计学意义(P<0.05)。结论IFN-γ+874单核苷酸多态性与婴儿巨细胞病毒肝炎的易感性及恢复有一定的相关性,IFN-γ+874AA型患者易感染巨细胞病毒,治疗后恢复慢。

Objectives To determine the association of interferon （IFN） -γ ＋874 gene polymorphism with infant cytomegalovirus hepatitis. Methods Eighty-seven infants with cytomegalovirus hepatitis and 89 children without cytomegalovirus infection were included in this study. Gene polymorphism of IFN-γ promoter at 874 A/T was determined by TaqMan fluorescence polymerase chain reaction. Results The frequencies of IFN-γ ＋874 AA, AT, TT genotypes were 64, 20, 3 and 45, 26, 18 in the study and control groups respectively. There was significant difference between two groups （P 〈 0.01） . The frequencies of IFN-γ ＋874 AA, AT, TT genotypes were 43, 19, 3 and 23, 1, 0 in the successfully treated and unsuccessfully treated groups respectively. There was significant difference between two groups （P 〈 0.05） . Conclusions Gene polymorphism of IFN-γ promoter at 874 A/T was correlated with the susceptibility and recovery of cytomegalovirus hepatitis. Infants with IFN-γ ＋874 AA may be more susceptible to cytomegalovirus infection and may have a slow recovery if they are infected.