GC法对中药材中残留农药的多残留分析

Determination of Organochlorine and Pyrethroid Pesticide Residues in Chinese Herbal Medicines by Capillary Cas Chromatography

建立了中药材中8种有机氯农药和4种拟除虫菊酯农药残留气相色谱分析方法。样品用丙酮-石油醚超声提取,再用正已烷进行液-液分配,提取液用弗罗里硅土柱净化,采用SE-54弹性石英毛细管柱分离,用GC-ECD同时检测。测定结果高、中、低3个水平添加时的回收率为80.6%-119.8%,相对标准偏差为0.7%-9.5%。该方法的检出限为：有机氯农药0.0005-0.007mg/kg,拟除虫菊酯0.005-0.008 mg/kg。通过实验结果显示本方法可用于中药材中农药残留测定。

Method to determine organochlorine and pyrethroid pesticide residues in Chinese herbal medicines by GC was found. Pesticide residues were extracted from Chinese herbal medicines with acetone and petroleum ether, Liquid - liquid extraction was performed with n - hexane and the clean - up of the extracts were made with alumina neutral - florisil column. Analytical screening was determined by gas chromatography using capillary column equipped with electron - capture detector. The recovery obtained from Chinese herbal medicines samples （ n = 3 ） at three concentration levels was in the ranges of 80.6% -119.8%, with relative standard deviations of 0.7% -9. 5%. Detection lii..it was from 0. 00005 to 0.0007 mg/kg for organochlorines and 0. 005 to 0.008mg/kg for pyre- throids respectively. The results show that this method can be used to determine organochlorine and pyrethroid residues in Chinese herbal medicines.