摘要
利用光镜、透射电镜、凝胶电泳和原位末端标记技术(TUNEL)对鸡新城疫病毒(NDV)F48E8株在体内诱导鸡胸腺淋巴细胞的凋亡情况进行了观察和检测,以期探讨NDV的致病机制。经NDV感染后,鸡胸腺淋巴细胞呈现典型的凋亡特征,感染组胸腺的淋巴细胞凋亡率显著高于对照组(P<0.01)。结果表明:NDV标准强毒F48E8株可以在体内诱导胸腺淋巴细胞凋亡,由此所致的免疫抑制可能是NDV致病的重要原因。
Light microscope, transmission electron microscope, electrophoretic analysis of DNA fragmentation and terminal deoxynucleotidyl transfers （TdT） dUTP nick and labeling （TUNEL） were used to study the apoptosis of chicken thymus lymphocytes induced by Newcastle Disease Virus （NDV） F48E8 in vivo. The results showed that chicken thymus lymphocytes exhibited obvious apoptosis. Moreover the apoptotic index of infected group was significantly higher than that of the control group （P 〈 0. 01 ）. It suggested that NDVF48E8 can induce apoptosis of chicken thymus lymphocytes in vivo, which will lead to immunodepression and may be the most important pathogeny.
出处
《畜牧与兽医》
北大核心
2007年第4期17-19,共3页
Animal Husbandry & Veterinary Medicine
基金
广西自治区自然基金项目(桂科自0640021)
关键词
新城疫病毒
胸腺
细胞凋亡
淋巴细胞
Newcastle disease virus
thymus
apoptosis
lymphocytes
