改变组织醛固酮及其受体表达对SHR大鼠肾脏纤维化的影响

Effects of Changing Levels of Renal Aldosterone and Its Receptor on Renal Fibrosis in Spontaneously Hypertensive Rats

【目的】观察改变肾组织醛固酮及其受体水平对自发性高血压大鼠肾脏纤维化的影响。【方法】8周龄的雄性自发性高血压大鼠(SHR)24只及同源正常京都大鼠(WKY)8只,SHR分为贝那普利干预组[30mg/(kg·d)]、大剂量安体舒通干预组[100mg/(kg·d)]和高血压模型对照组,同时设同源的正常对照组,干预时间为8周,检测收缩压、尿蛋白、血白蛋白、尿素氮、肌酐、肾组织醛固酮受体、TGF-β1的mRNA和蛋白表达。【结果】贝那普利能下调组织醛固酮[(15.8±2.6)vs(22.2±0.6)pg/mg]及其受体水平[(15±4)vs(13±5)PU]并减轻肾脏纤维化[(17.0±1.8)vs(20.0±2.3)PU],均为P<0.05;大剂量安体舒通能上调组织醛固酮[(24.3±4.6)vs(22.2±0.6)pg/mg]及其受体水平[(16±6)vs(13±5)PU]并加重肾脏纤维化[(22.6±3.0)vs(20.0±2.3)PU],均为P<0.05。【结论】肾组织醛固酮及其受体水平的改变可能影响高血压肾脏纤维化的过程。

[Objective ] To investigate the effects of different levels of renal aldosterone and its receptors in renal fibrosis in spontaneously hypertensive rats （SHR）. [Methods] Twenty-four 8-week-aged male SHRs were divided into three groups： spironolactone group [ 100 mg/（kg·d）], benapril group [30 mg/（kg·d）], hypertension model group. The authors also observed the normal group （eight 8-week-aged male Wistar-Kyoto rats）. After eight weeks interference, the authors checked systolic blood pressure, proteiuria, albumin, blood urea nitrogen, and creatinine, the expression of mineralocorticoid receptor and transforming growth factor beta 1 （TGF-β1） in renal tissue. [Results] Benapril down-regulated the levels of renal aldosterone [（15.8＋2.6） vs （22.2＋0.6） pg/mg] and its receptors [（15±4） vs （13±5） PU], alleviate renal fibrosis [（17.0±1.8） vs （20.0±2.3） PU], all were P〈 0.05, while spironolactone up-regulated the levels of renal aldosterone [（24.3±4.6） vs （22.2±0.6） pg/mg] and its receptor [（16±6） vs （13±5） PU] and aggravated renal fibrosis [（22.6±3.0） vs （20.0±2.3）PU], all were P〈 0.05.[Conclusion]The change of aldosterone and its receptors in kidney can affect the process of renal fibrosis in SHR.