摘要
在pH1.64的Clark-Lubs缓冲溶液中,牛血清白蛋白与酸性黄作用时导致吸收光谱发生变化,在532 nm附近吸光度明显降低,而在424 nm附近吸光度则呈增强趋势,其︱ΔA︱-1与cP-1呈良好线性。本文系统研究了酸性黄与蛋白质的作用机理及实验条件对酸性黄与蛋白质结合反应的影响,探讨了蛋白质与酸性黄之间的作用力。本法应用于人尿中总蛋白的测定,与经典的考马斯亮蓝G-250法结果一致。
In a Clark - Lubs buffer solution of pH 1.64, acid yellow reacts with protein to form a complex resulting in a decrease of absorbance around λ532 nm and an increase of absorbance around λ424 nm Experimental results showed that the plot of ｜△A ｜^ - 1 vs. Cp ^-1 （ concentration of bovine serum albumin） was linear with a correlation coefficient value of 0. 996. The interaction mechanism and the binding force between acid yellow and protein were studied by UV spectra. The absorption characteristics of the complex formed in various conditions was also studied. The method has been used for the determination of total protein in human urine sample and the results obtained agreed well with those obtained by coomassie brilliant blue G-250 method.
出处
《分析测试学报》
CAS
CSCD
北大核心
2007年第4期541-544,共4页
Journal of Instrumental Analysis
关键词
蛋白质
酸性黄
作用机理
人尿
Protein
Acid yellow
Reaction mechanism
Human urine
