摘要
基于在盐酸介质中磺胺醋酰钠-亚硝酸钠-1-胺的重氮化偶合反应,利用顺序注射技术在512nm波长处监测不稳定的红色产物,建立了快速、自动测定磺胺醋酰钠的新方法。考察了影响化学反应的因素,并对反应机理进行了初步探讨。方法的线性范围为2~260mg/L,检出限为1.03mg/L,进样频率为100次/h。应用于磺胺醋酰钠滴眼液的测定,结果与药典规定方法对照,并经甜佥验证明,两种方法无显著性差异。
Based on the diazotisation of sulfacetamide sodium (SAS) with sodium nitrite and coupling with 1-naphthylamine in 1.0 × 10^-2 mol/L HCl, a rapid and sensitive spectrophotometric method for the determination of SAS with sequential-injection analysis was proposed. The absorbance of the unstable red product of the reaction was measured at 512 nm. The linear range of the calibration curve for SAS was 2-260 mg/L, the detection limit was 1.03 mg/L, and the sample throughput was 100/h. The method has been used for the determination of SAS in eye drops of SAS. The results were statistically compared with those obtained by pharmacopoeia method.
出处
《分析测试学报》
CAS
CSCD
北大核心
2007年第4期552-555,共4页
Journal of Instrumental Analysis
基金
河南省自然科学基金资助项目(0511021200)
关键词
磺胺醋酰钠
顺序注射分析
重氮偶合
动力学光度法
Sulfacetamide sodium
Sequential-injection analysis
Diazo coupling
Kinetic spectropho-tometry