摘要
目的:建立HPLC法测定头孢克洛胶囊含量。方法:色谱柱:Cosmosil ODSC18柱(4.6mm×250mm,5μm),流动相:磷酸二氢钾溶液-乙腈(90:10),流速:1mL/min,检测波长:254nm,进样量20μL。结果:头孢克洛在0.0564~0.2820g/L浓度范围内线性关系良好,r=0.9998,平均回收率为99.26%,RSD为0.81%(n=9)。结论:该方法快速、灵敏、准确、可靠,适用于头孢克洛胶囊的含量测定。
Objective: To establish a HPLC method for the determination of content of cefaclor capsules. Methods: Cosmosil ODS C18 column(4.6mm × 250mm,5μm) and potassium dihydrogen phosphate solution- acetonitrile (90:10) as the mobile phase were used at a flow rate of 1 mL/min,with a detection wavelength of 254 nm and a injection volume of 20μL. Results: The calibration curve was liner in the range of 0.0564 - 0.2820 g/L, r = 0.9998. The average recovery was 99.26% (RSD = 0.81%, n = 9). Conclusion: The method is rapid, sensitive, accurate, reliable and suitable for determination of content of cefaclor capsules.
出处
《药学进展》
CAS
2007年第7期320-322,共3页
Progress in Pharmaceutical Sciences