参附注射液对肝缺血再灌注大鼠血浆前列环素和血栓素A_2及肝组织ATP酶的影响

Effects of Shenfu Injection on prostacyclin, thromboxane A_2 and activities of ATPases in rats exposed to hepatic ischemia-reperfusion injury

目的:探讨参附注射液对大鼠肝缺血再灌注损伤的保护作用及其机制。方法:24只Wistar大鼠随机分为模型组和参附注射液(Shenfu Injection,SF)治疗组。SF治疗组大鼠腹腔注射参附注射液10ml/kg。模型组大鼠给予相同剂量的生理盐水。两组均采用Pringle's法阻断肝门缺血15min再灌注1h和3h,测定血浆血栓素B2(thromboxane B2,TXB2)和6-酮-前列腺素F1α(6-keto-prosta-glandin F1α,6-keto-PGF1α)以及肝组织匀浆Na+-K+-ATP酶、Ca2+-Mg2+-ATP酶的变化,并观察肝组织形态学改变。结果:再灌注3h SF治疗组血浆TXB2低于模型组,6-keto-PGF1α高于模型组,两者比值TXB2/6-keto-PGF1α低于模型组;再灌注1h、3h SF治疗组Na+-K+-ATP酶和Ca2+-Mg2+-ATP酶活性高于模型组。SF治疗组肝实质细胞和线粒体损伤明显减轻。结论:参附注射液对肝缺血再灌注损伤有保护作用,其机制与降低TXA2/PGI2比值,提高Na+-K+-ATP酶和Ca2+-Mg2+-ATP酶活性有关。

Objective： To explore the effects of Shenfu Injection on prostacyclin, thromboxane A2 and the activities of ATPases in rats exposed to hepatic ischemia-reperfusion injury. Methods： Twenty-four male Wistar rats weighing 200-250 g were randomly divided into two groups： Shenfu Injection （SF）-treated group （rats were treated with Shenfu Injection of 10 ml/kg through intraperitoneal injection） and untreated group （rats were administered with normal saline at the same dose and served as a control group）. Hepatic ischemia was caused by Pringle＇s maneuver and lasted for fifteen minutes, and then one-hour or three-hour reperfusion was performed. Venous blood samples for the measurement of thromboxane B2（TXB2） and 6-keto-prostaglandin F1α （6-keto-PGF1α） were collected three hours after reperfusion. Liver tissue samples were collected one hour or three hours after reperfusion for the measurement of Na^＋-K^＋- ATPase and Ca^2＋-Mg^2＋-ATPase and for morphological studies. Results： Plasma TXB2 was lower in the SF-treated group than that in the untreated group after three-hour reperfusion （P〉0. 05）, while 6-keto-PGF1α was higher in the SF-treated group than that in the untreated group （ P〉0. 05）. The ratio of TXB2 and 6-keto-PGF1α was significantly lower in the SF-treated group than that in the untreated group （P〈0. 05）. The activities of Na^＋-K^＋-ATPase and Ca^2＋-Mg^2＋-ATPase in the SF-treated group were improved obviously. A three-hour reperfusion after fifteen-minute ischemia caused important hepatic histological alterations. Marked structural abnormalities were observed in the untreated group, such as massive hepatocyte swelling, necrosis, mitochondria edema and vacuolar changes. In the SF-treated group, hepatic tissue injury was reduced significantly. Conclusion： Shenfu Injection protects hepatic tissue from ischemia-reperfusion injury, and such protective effects are achieved by decreasing the ratio of thromboxane A2 and prostacyclin, and increasing the activities of Na^＋-K^＋-ATPase and Ca^2＋-Mg^2＋- ATPase.