益气消积方含药血清对人胃癌NKN-28细胞株的作用

Effects of serum containing Yiqi Xiaoji Recipe on cell line NKN-28 of gastric cancer

目的:研究益气消积方含药血清对人胃癌NKN-28细胞株的作用。方法:以SD大鼠为实验血清的供体,将大鼠血清灭活,以灭活前给药与否及给药剂量分为高、中、低剂量组及空白血清组,以细胞抑制率为观察指标,对实验结果进行分析。结果:4组大鼠血清对NKN-28细胞株生长均有抑制作用,且对NKN-28细胞株生长的抑制作用具有一定的浓度依赖性;实验组各浓度组间差异有统计学意义(P<0.01)。结论:益气消积方含药血清可明显抑制NKN-28细胞株的生长,抑制作用呈一定的浓度依赖性。

Objective. To explore the effects of serum containing Yiqi Xiaoji Recipe （YQXJR）, a compound traditional Chinese herbal medicine for benefiting qi and removincl stagnation, on cell line NKN-28 of gastric cancer. Methods： The experimental SD rats were taken as the provider of the animal serum, and the serum was inactivated before the experiment. The serum was divided into high-dose, medium-dose, low-dose and blank serum groups based on whether the rats were given YQXJR and administration dosage. The inhibition rate was regarded as the observational index. Results： The four groups of serum all had inhibitory effect on the growth of NKN-28 cells depending on the drug concentration. And there were significant differences among the experimental groups. High-dose, mediumdose and low-dose concentrations of serum all could inhibit the growth of NKN- 28 cells with positive relations with the concentration and function time. Conclusion： YQXJR serum can inhibit the growth of NKN-28 cells depending on the drug concentration and function time.