摘要
本文应用抗增殖细胞核抗原的单克隆抗体ＰＣ１０，用ＬＳＡＢ免疫组化方法对４０例胃癌标本进行研究。结果发现：胃癌不同分化程度各组中ＰＣＮＡ指数存在差异（Ｐ＜０．０２），低分化腺癌＞中分化腺癌＞高分化腺癌；淋巴结癌转移阳性组ＰＣＮＡ指数显著高于无淋巴结转移组（Ｐ＜０．０５）；癌细胞浸润达肌层、浆膜层者ＰＣＮＡ指数显著高于粘膜内癌（Ｐ＜０．０１）。结果显示：此方法是在石蜡切片上检测胃癌增殖指数的一个有效手段，并对判断胃癌患者的预后有一定作用。
An immunohistochemical study of gastric carcinoma using PC10,a monoclonal antibody against proliferating cell nuclear antigen (PCNA),was performed on paraffin Sections, There was significant difference in PCNA index between any two groups of different differentiation;PCNA indexes in the group of lymph node metastas and in the group of invasion to muscle and serosa layer were higher than those of no lymph node metastasis or carcinoma in mucoma. The result indicated that PCNA detection was a reliable method to examine the fraction of proliferating cells of gastric carcinoma on paraffin sections ,and it could be used to determine the prognosis of gastric carcinoma.
