玉竹基因组DNA的提取与分析

Extraction and analysis of DNA in Polygonatum odoratum

目的以玉竹根茎为材料,寻找一种适合于玉竹总DNA提取的方法,便于下游分子生物学的操作。方法采用经典CTAB法与改良CTAB法。结果改良CTAB法提取得到的DNA可以很好地用于PCR扩增。结论改良CTAB法适用于类似玉竹这类含多糖较多的根及根茎类中药材DNA的提取。

Objective To establish a extraction method for high-quality DNA from Polygonatum odoratum . Method The samples were extracted by the classic technique of CTAB and improved CTAB. Results DNA extracted with improved CTAB from fresh samples of Polygonaturn odoratum was successfully amplified with PCR technique. Conclusion The improved CTAB technique can be used for extracting DNA from herbs riching polysaccharide.