Ⅳ型胶原放射免疫分析的研究

RADIOIMMUNOASSAY FOR TYPE Ⅳ COLLAGEN AND ITS CLINlCAL APPLlCATlON

目的：建立Ⅳ型胶原（ＣＩＶ）测定的放射免疫分析法。方法：从人胎盘组织提纯ＣＩＶ，免疫兔获得特异抗血清，制备１２５￣Ⅰ－ＣＩＶ标记抗原并建立ＣＩＶ放免分析法。用此法检测９０名正常人和１５０例各种肝病患者血清ＣＩＶ含量。结果：该法标准曲线工作范围２５～８００μｇ／Ｌ，灵敏度１０μｇ／Ｌ，批内和批间变异系数分别为４．７％和６．３％，平均回收率９５．０％。用该法测定正常人血清ＣＩＶ含量为９２．１±１１．０μｇ／Ｌ：慢活肝，肝硬化和原发性肝癌组血清ＣＩＶ水平显著高于正常组（Ｐ值＜０．０１）；急性肝炎组略有升高。慢活肝，肝硬化和原发性肝癌组高于急性肝炎和慢迁肝组（Ｐ值＜０．０１）。结论：建立的ＣＩＶ放射免疫分析法具有简便、灵敏、特异的优点，对肝纤维化及肝硬化的诊断具有临床实用价值。

Aim: A radttoimmunoassay (RIA) was developed for the determination of type Ⅳ collagen (CIV). Methods: GIV was pudfied from human plaomta and used to raise antiserum in rabbits. 125Ⅰ-labeled CIV was prepared. The RIA for GIV was developed and applied to 90 healthy subjects and 150 patients with various liver diseases. Results: This assay with a working range from 25mg/L to 800mg/L provided a sensitivity of 10mg/L. The coefficients of variation of intra-and inter-assay were 4. 7% and 6.3%, respec- tively. the average recovery rate was 95.0%. As compared with healthy subjects (92.1± 11.0mg/L), the serum levels of CN were signicantly elevated in patients with chronic active hepatitis, cirrhosis and hepatocellular carcinnoma (P<0.01 ); Patients with acute hepatitis showed a dightly high serum CIV level: patients with chronic active hepatitis, cirrhosis and hepatocellular carcinoma had significantly higher serum concentrations of CIV than thoae with acute hepatitis and chronic perdstent hepatitis (P<0.01 ). Conclusion : The RIA for serum CIV is simple. sensitive, accurate and slecific,and provides a useful method for clinical . diagnosis of hepatic fibrosis and cirrhosis.