慢性丙型肝炎患者血清HCV　RNA定量分析──竞争性聚合酶链反应

QUANTITATIVE ANALYSIS OF SERUM HEPATITIS C VIRUS RNA IN pATIENTS WITH CHRONIC HEPATITIS C BY COMpETITIVE REVERSE TRANSCRlPTION-POLYMERASE CHAIN REACTION

目的：探讨ＨＣＶ复制水平与肝病进展的关系。方法：用竞争性逆转录聚合酶链反应（ＣＲＴ－ＰＣＲ）定量检测２７例慢性丙肝患者（ＣＨＣ）血清ＨＣＶＲＮＡ。结果：ＣＨＣ患者血清ＨＣＶＲＮＡ水平变化范围１０２～１０６ｃｏｐｉｅｓ／５０ｕｌｓｅｒｕｍ。１３例慢性迁延性肝炎（ＣＰＨ）患者血清ＨＣＶＲＮＡ平均水平（５．０６８±１．０４，Ｌｏｇ１０ｃｏｐｉｅｓ／５０μｌｓｅｒｕｍ）明显低于１０例慢性活动性肝炎患者（ＣＡＨ）（５．７９±０．２５）和４例肝硬化（Ｃｉｒ）患者（５．８３±０．７５），Ｐ值均＜０．０５。血清ＨＣＶＲＮＡ水平同ＡＬＴ水平间呈正相关（ｒ＝０．４９９７，Ｐ值＜０．０５）。结论：表明丙肝病毒感染后病毒血症水平低，ＨＣＶ复制水平与肝损伤相关，在慢性感染中ＨＣＶ仍活跃复制，并与肝病进展相关。

Aim : Quantitative analysis of serum hepatitis C virus RNA in patients with chronic hepatitis. Methods: The levels of serum HCV RNA in 27 chronic hepatitis C patients were investigated with Com- petitive Reverse Transcription-Polymerase Chain Reaction (GRT-PCR). Results : The amounts of circulating HCV RNA ranged from 102 to106 copies/50.l serum in the 27 patieatts. The average amount of circulating HCV RNA in I 1 3 patients with chronic persiutent hepatitis(1 0s.. . t,.) was si.ficantly less than those in 1 0 chronic active hepatitis or cirrhosis( 105.79±0.25 , and 105.83±0.75,, copies/50μl serum, respectively (P<0.05). The amounts of HCV RNA in serum were correlated to the levels of ALT(r=0.4997, P<0.05). Conclusion: The replication of HCV may occur more active in the advanced stages than that in the early stages of type C patients.