TIMP-2和VEGF表达与喉癌预后关系

Relationship Between the Expression of TIMP-2 and VEGF in Squamous Cell Carcinoma of Larynx and the Prognosis of Patients with Laryngnocarci Noma

目的探讨组织基质金属蛋白酶抑制剂2(TIMP-2)和血管内皮生长因子(VEGF)与喉鳞状细胞癌预后的关系。方法用EnVision免疫组化方法检测38例喉鳞状细胞癌及19例喉癌旁正常组织中TIMP-2和VEGF的表达;术后随访3～5年,患者中局部复发4例,颈淋巴结转移2例,肺部转移1例。结果喉鳞状细胞癌组织中TIMP-2和VEGF的表达水平高于癌旁正常组织(P<0.05),无瘤生存的喉癌组织中VEGF的表达水平及VEGF/TIMP-2的比值均高于术后复发转移组(P<0.01),TIMP-2的表达水平前组低于后组(P<0.01)。结论TIMP-2和VEGF的表达水平及两者的比值与喉鳞状细胞癌的预后有关。

Objective To study the correlation between expression of vascular endothelial growth factor （VEGF） and tissue inhibitor of metalloproteinase 2 （TIMP - 2） and prognosis of patients with laryngeal squamous cell carcinoma. Methods The expression of VEGF and TIMP - 2 were measured in 38 specimen of LSCC and 19 specimens of normal laryngeal tissue adjacent to the tumors with EnVision immunohistochemical technique, follow - up for 3 - 5 years after operation local relapse occurred in four cases, cervical nodular metastasized occurred in fwo cases and pneumonic metastasis occurred one case. Results The expression level of two marks in primary tumors was significantly higher than that in pericancerous tissues （P 〈 0.05 ） , significant correlation was found between two marks in laryngeal squamous cell carcinoma（ rs = 0. 558 ,P 〈 0.05 ）. The expression of VEGF and the ratio of VEGF/TIMP - 2 in laryngeal carcinoma tissue of patients with better prognosis were higher than that in tissue of patients with poorer prognosis（ P 〈0.01 ）. The expression of TIMP - 2 in anterior tissue is lower than that in latter tissue （P 〈 0.01 ）. Conclusions The expression of VEGF and TIMP - 2 and the ratio of two marks are three value indexes for prognosis of patients with laryngeal carcinoma.