阿月浑子组织培养研究初报

Preliminary Studies on Tissue Culture of Pistachio

阿月浑子是中国西部与华北地区极有发展前景的坚果树种,但其微繁技术至今未有突破,以7年生阿月浑子嫁接树茎段为外植体,进行启动培养试验,对MS、改良MS、Q-L4、DKW 4类培养基,BA、IBA、NAA、GA 4种激素与用量,7%次氯酸钠和0.1%升汞溶液与灭菌时间、葡萄糖和蔗糖与用量、叶酸、D-泛酸钙与LH(水解乳蛋白)3种添加剂与用量的培养效果进行比较分析,确定阿月浑子外植体0.1%升汞溶液灭菌10min效果最好,最优培养基为1/2DKW+0.5mg/L 6-BA+0.5mg/LIBA+mg/L GA3+1mg/L叶酸+300mg/L LH+1mg/l D-泛酸钙+15g/l葡萄糖+6.5g/l琼脂。

Pistachio is a potential nut tree in the north and west of China, however, the micropropagation of pistachio is not still successful, this experiment were designed for establishing a tissue culture techniques of pistachio. Shoots collected from grafted tree （7yrs）of pistachio were used as explant in primary culture with different treatments of medium（MS, modified MS,Q-L4,DKW）, plant hormone（BA,IBA, NAA,GA）, disinfectant （7%NaC103, 0.1%mercuric chloride）, addition （folic acid, calcium D-pantothenate and LH）, glucose and sucrose, experiment results shown that explant of pistachio were disinfected well with 0.1% mercuric chloride for 6-BA ＋0.5mg/L IBA ＋3.0 mg/L 10min, the suitable primary medium for pistachio was： 1/2DKW＋0.5mg/L GA3＋1mg/L folic acid＋ 300mg/L LH ＋lmg/L calcium D-pantothenate＋15g/L glucose＋6.5g/L agar.