亚精胺处理青稞幼苗对NaCl胁迫作用的缓解

Amelioration of NaCl Stress by Spermidine Pretreatment in hulless barley Seedlings

以青稞(Hordeum vulgare L.var.nude)幼苗叶片为试材,以200mmol/L NaCl对其进行盐胁迫,研究了外源亚精胺(Spd)预处理对NaCl胁迫下青稞幼苗叶片相对含水量(RWC)、叶绿体色素与水溶性蛋白质含量的影响。在NaCl胁迫的前两天,NaCl+Spd处理组用含0.5mmol/L Spd的1/2Hoagland营养液进行水培和叶面喷施含0.02%土温-80的0.5mmol/L Spd溶液;对照组(CK)和NaCl处理组,用1/2的Hoagland营养液进行水培,叶面喷施含0.02%土温-80的蒸馏水。随着200mmol/L NaCl胁迫的进行,青稞幼苗叶片RWC、叶绿体色素与水溶性蛋白质含量均明显降低,Spd预处理显著延缓了它们的下降。表明Spd预处理缓解了NaCl胁迫对青稞幼苗造成的伤害,具有防衰抗盐的保护作用。

The effects of spermidine pretreatment on relative water content （RWC）, contents of chloroplast pigment and soluble protein in leaves of hulless barley seedlings （Hordeum vulgare L. var. nude）under 200mmol/LNaC1 stress were studied. Seedlings of control and NaCl treatment were daily sprayed with distilled water ＋ 0.02% （V/V） Tween-80 and irrigated with half-strength Hoagland＇s nutrient solution, and Seedlings of Spd pretreatment were daily sprayed with 0.5 mmol/L Spd ＋ 0.02 % （V/V） Tween-80 and irrigated with 0.5 mmol/L Spd ＋ half-strength Hoagland＇s nutrient solution for 2 days before NaCl stress. Under 200mmol/L NaCl induced stress, RWC, levels of chloroplast pigment and soluble protein in leaves of hulless barley seedlings obviously decreased, and preliminary supply of Spd could distinctly alleviate their decline. These results suggested that using of exogenous spermidine could obviously alleviate the injury of NaCl stress, and have protective effects on delaying senescence and resisting NaCl stress of hulless barley seedlings.