小麦抗叶锈基因Lr38差异表达的初步研究

Preliminary Study on Differential Expression of Wheat Leaf Rust Resistance Gene Lr38

小麦叶锈病是一种世界性发生的小麦病害,利用抗病品种是防治该病害最经济、安全、有效的方法。目前已经正式定名的56个小麦抗叶锈基因中,有许多表现出抗锈性的丧失。来自中间偃麦草的小麦抗叶锈基因Lr38则表现出优良的抗锈性。利用DDRT-PCR技术,分析了小麦抗叶锈近等基因系材料TcLr38与感病对照Thatcher间的表达差异。通过对差异片段的克隆测序,获得一条425bp的差异表达序列,推测可能为一与抗病相关的基因片段。

Leaf rust is one of the most important diseases in wheat throughout the world. The use of resistant cultivars is the most economical, effective and safest method in leaf rust disease control. Up to date now, 55 leaf rust resistance genes have been formally named, but most of them have lost resistance against leaf rust. The resistance gene Lr38 derived from Agropyron intermedium, expressed high resistant levels for leaf rust disease. The study analyzed the differentially expressed bands between the leaf rust resistance gene Lr38 and Thatcher. A 425 bp band had been found by cloning and sequencing, which maybe related with the leaf rust resistance gene.