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家蚕Bm-mrg15基因的克隆与序列分析

Cloning and Sequence Analysis of the gene Bm-mrg15 in Bombyx mori
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摘要 含MRG结构域的基因大多在基因的转录调节中起着重要作用.利用电子克隆,在家蚕中得到了一个含MRG结构域的基因Bm-MRG15,并对其进行了克隆测序和序列分析.该基因cDNA长1113bp,有6个外显子,编码区长1020bp,序列的1091bp处有2个重叠的poly(A)加尾信号.推定的蛋白质编码339个氨基酸,N端含有一个染色质域,序列内有5个保守的结构域.RT-PCR显示该基因在所检测家蚕的各时期和组织中均有表达. Most of the genes containing MRG domain play key roles in gene trascription. A MRG domaincontaining gene, Bm-mrglS, is obtained by electronic clone and experimental validation in silkworm （Bombyx mori）. The results show that the gene contains 6 exons, and the length of its cDNA and ORF are 1 113 bp and 1 020 bp, respectively. There are two overlapped poly（A） signals at mRNA 1 091 bp. The deduced protein is 339aa, with a full sequence. The transcript activity RT-PCR. chromodomain in the N-terminal and five conserved domains in the can be detected in all tissues and at all development stages tested by
作者 孔卫青 杨金宏 朱勇
机构地区 西南大学蚕学与生物技术学院
出处 《西南大学学报（自然科学版）》 CAS CSCD 北大核心 2007年第6期56-61,共6页 Journal of Southwest University(Natural Science Edition)
基金 重庆市教委基金资助项目(040208).
关键词 家蚕 MRG域 染色质域 外显子/内含子 Bombyx mori MRG domain chromodomain exon/intron
分类号 S851.347.31 [农业科学—预防兽医学]
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参考文献17

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西南大学学报（自然科学版）
2007年 第6期

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