特发性脊柱侧凸患者椎旁肌中神经营养素3的表达及意义

Neurotrophin-3 mRNA expression in paravertebral muscles of patients with idiopathic scoliosis

目的:研究特发性脊柱侧凸(IS)患者椎旁肌组织中神经营养素3(Neurotrophin-3,NT-3)在核酸水平(mRNA)的表达情况。方法:随机选择19例IS患者,年龄12～19岁,平均14.3岁,Cobb角42°～80°(平均54°)。手术中取顶椎(T7～T10)凸侧、凹侧椎旁肌肉组织,采用半定量RT-PCR法检测NT-3 mRNA表达水平。取4例相同年龄段的椎间盘突出患者手术切口头端非病变部位椎旁肌肉组织作为对照。结果:对照组椎旁肌中NT-3的mRNA表达阳性率50%,相对表达量为0.0237±0.0158,IS组患者椎旁肌中表达阳性率63.16%,相对表达量为0.1213±0.0939,较对照组表达量增加(P=0.051)。9例Cobb角>50°的IS患者表达阳性率为44.44%,相对表达量0.0431±0.0359,和对照组无明显差异;10例Cobb角≤50°的IS患者中表达阳性率为80%,相对表达量为0.1604±0.0895,与对照组和Cobb角>50°的IS患者比较有明显增加(P<0.01)。结论:正常椎旁肌组织中NT-3在核酸水平微量表达,特发性脊柱侧凸患者椎旁肌组织中NT-3 mRNA表达增加,尤其在小角度IS患者中增加明显,提示IS患者中存在神经营养因子表达异常。

Objective：To investigate Neurotrophin-3（NT-3） mRNA expression in paravertebral muscles in idiopathic scoliosis （IS）.Method：Muscle samples were harvested from 19 female IS patients （mean age 14.3 years,range 12-19 years） with Cobb angle from 42°-80°（average 54°）.Muscle biopsies were taken bilaterally from the deep paravertebral muscle at the apex of the curve（T7-T10） during surgery,which was snap frozen in -80℃ and processed for semi-quantitation RT-PCR.Muscle biopsies were taken from two females and two male age-matched patients without scoliosis who were underwent spinal surgery for lumbar intervertebral disc herniation as control group.Result：Compared with control group,which had low level NT-3 mRNA expression （positive rate was 50% and comparative expression was 0.0237±0.0158）,a increase of NT-3 mRNA expression （positive rate was 63.16% and comparative expression was 0.1213±0.0939） was found in IS group （P=0.051）.The expression level of NT-3 mRNA in the patients with Cobb angle ≤50° （positive rate was 80% and comparative expression was 0.1604±0.0895） was statistical higher than that in the patients with the Cobb angle〉50° or normal （P〈0.01）.Conelusion：There is increasing expression of NT-3 mRNA in the paravertebral muscle in idiopathic scoliosis.