化妆品中氯霉素的气相色谱-质谱快速分析

Study on rapid analysis of chloramphenicol in cosmetics by GC/MS

研究了用气相色谱．质谱分析化妆品中氯霉素（CAP）的方法。以乙酸乙酯作为提取溶剂，BSTFA＋TMCS（99＋1）作为衍生化试剂，在70℃衍生化60min，用GC—MS（NCI）进行测定。方法的检出限为10μg/kg，线性范围在0．5～500ng/mL之间，在10、100、500μg/kg 3个添加水平，方法回收率为94．4％～95．7％，相对标准偏差为0．23％～1．3％。方法操作快速简单，3h可以完成整个样品分析。方法已用于化妆品中氯霉素的测定。

A method for the analysis of chloramphenicol in cosmetic by gas chromatography （GC）/mass spectrometry （MS） （negative chemical ionization） （NCI） was studied. The optimum conditions are as follows： using acetic ether as an extractant and BSTFA ＋ TMCS （99 ＋ 1 ） as derivative reagents, chloramphenicol was derived at 70 ℃ for 60 min, and then determined by GC/MS （NCI）. The detection limit is 10 μg/kg, and the linear range is from 0.5 to 500 ng/mL. The recoveries are 94.4% -95.7% and the relative standard deviations are 0.23% - 1.3%. The method is simple and rapid, and the samples can be finished the analysis within 3 h. The method has been applied to the determination of chloramphenicol in cosmetics.