毛细管电泳法测定银黄胶囊及银黄颗粒中黄芩苷和绿原酸的含量

Determination of baicalin and chlorogenic acid in Yinhuang capsules and Yinhuang granules by capillary electrophoresis

目的建立同时测定银黄胶囊及银黄颗粒中黄芩苷和绿原酸含量的方法。方法采用毛细管电泳法，电泳条件：未涂层弹性融硅石英毛细管柱（55cm×75μm ID，有效长度47cm）；以40mmol／L硼砂溶液（pH9．30）为运行缓冲液；分离电压15kV；重力进样5s（高度15cm）；检测波长310nm。结果以对硝基苯甲酸为内标，黄芩苷、绿原酸的线性范围分别为20．2～101．0μg／mL（r=0．9981），5．15—25．75μg／mL（r=0．9991）。银黄胶囊中黄芩苷、绿原酸的平均回收率分别为105.0％、97．2％，银黄颗粒中黄芩苷、绿原酸的平均回收率分别为101．3％、96．6％。结论该法简便、快速、准确可靠，可用于银黄胶囊及银黄颗粒的质量控制。

Objective To establish a method for the content determination of baicalin and chlorogenic acid in Hnhuang capsules and Yinhuang granules. Methods Samples were analyzed on a uncoated fused silica capillary （55 cm ×75 μm, 47 cm） using 40 mmol/L Na2B4O7（pH 9.30） as running buffer. The voltage was 15 kV. The detection wavelength was 310 nm. Results The method was validated over linear ranges of 20.2 - 101 μg/mL（ r = 0. 9981 ） for baicalin and 5.15 - 25.75 μg/mL（ r = 0. 9991 ） for chlorogenic acid. The average recoveries of baicalin and chlorogenic acid in Yinhuang capsules were 105.0% and 97.2% , respectively. The average recoveries of baicalin and chlorogenic acid in Yinhuang granules were 101.3% and 96.6%, respectively. Conclusion This technique provides a sensitive, rapid, reproducible and inexpensive assay suitable for quantitative determination of baicalin and chlorogenic acid in Yinhuang capsules and Yinhuang granules.