RP-HPLC法测定轻身消胖丸中盐酸麻黄碱的含量

Determination of ephedrine hydrochloride in Qingshenxiaopang pills by RP-HPLC

目的建立轻身消胖丸中盐酸麻黄碱的定量方法。方法采用RP—HPLC法，色谱柱：Waters XTerra C18（4．6mm×250mm，5μm）不锈钢柱；流动相：1％磷酸溶液（含0．4％三乙胺）．乙腈（体积比91：9）；流速：1．0mL／min；检测波长：207nm；柱温：35℃。结果盐酸麻黄碱的进样量在16．6—166μg／mL范围内与峰面积呈良好线性关系，盐酸麻黄碱的平均回收率为98.40％（n=6），RSD为1．2％。结论方法简单快速、专属性强、重复性好、精密度高，可用于控制该制剂的质量。

Objective To establish an HPLC method for content detection of ephedrine hydrochloride in Qingshenxiaopang pills. Methods The sample was analyzed on a Waters XTerra C18 （4.6 mm - 250 mm, 5 μm） column using 1% H3PO4 （0.4% triethylamine）- acetonirile （91：9） as mobile phase. The detection wavelength was at 207 nm. Results The average recovery of the method was 98.4 % with RSD 1.2%. The linear range of phedrine hydrochloride was 16.6 - 166 μg/mL. Conclusion This method is simple, quick, reproducible and suitable for the quality control of Qingshenxiaopang pills.