慢性阻塞性肺疾病患者急性加重期血清基质金属蛋白酶抑制剂及基质金属蛋白酶9浓度与肺功能变化的关系

Relationship of matrix metalloproteinase-9 and tissue inhibitor of matrix metalloproteinase-1 with pulmonary function in the patients with chronic obstructive pulmonary disease

目的探讨慢性阻塞性肺疾病急性加重期(AECOPD)患者血清MMP-9、TIMP-1浓度的变化及其与肺功能变化的关系。方法应用酶联免疫吸附(ELISA)方法检测58例AECOPD患者和30名健康人血清中MMP-9、TIMP-1浓度。结果AECOPD患者血清MMP-9、TIMP-1浓度[(128.89±115.84)ng/mL、(228.28±107.133)ng/mL]明显高于对照组[(30.65±18.43)ng/mL、(133.69±41.41)ng/mL,P<0.01]。AECOPD组和对照组血清MMP-9、TIMP-1浓度与FEV1占预计值%具有负相关性(r=-0.591、-0.651,P<0.01);AECOPD组和对照组血清MMP-9、TIMP-1浓度与FEV1/FVC%具有负相关性(r=-0.558、-0.653,P<0.01);MMP-9/TIMP-1比率与FEV1占预计值%及FEV1/FVC%具有负相关性(r=-0.373、-0.356,P<0.05)。结论MMP-9、TIMP-1是引起肺功能下降很重要的因素。

Objective To investigate the change of matrix metalloproteinase （MMP） -9, tissue inhibitor of matrix metalloproteinase （TIMP） - 1 expression in the serum of patients with AECOPD as well as the correlation between the expressions of these factors and lung function. Methods The levels of serum MMP -9 and TIMP - 1 in 58 patients with AECOPD and 30 healthy donors were examined by enzyme - linked immunosorbent assay （ ELISA ）. Results The serum levels of MMP -9, TIMP - 1 （ 128. 89 ± 115.84） ng/mL, （ 228.28 ± 107.13 ） ng/mL in the patients with COPD were statistically significant higher than those of the control group （30.65 ± 18.43） ng/mL, （ 133.69 ± 41.41 ） ng/mL, P 〈 0.01. There was significantly negative correlation between the levels of MMP - 9, TIMP - 1 in serum and FEV1% （r= -0.591, -0. 651, respectively, P 〈 0.01）. This negative correlation was also seen between MMP-9, TIMP- 1 and FEVI/FVC% （ r = -0,558, -0. 653, respectively, P 〈 0.01 ）. Further- more, negative relationships were observed between the ratio of MMP- 9/TIMP- 1 and FEV1%, as well as MMP - 9/TIMP - 1 and FEV1/FVC% （ r = - 0. 373, - 0,356 respectively, P 〈 0.05 ）. Conclusion MMP -9 and TIMP - 1 may play an important role in the development of airway obstruction.