摘要
研究了8种树脂对青钱柳多糖的静态吸附性能,筛选出一种吸附较好的树脂,并利用柱层析进行青钱柳多糖的纯化研究。结果表明:D301R树脂对青钱柳多糖吸附量最大;D301R树脂在吸附液30℃,pH为7,浓度为4 mg/mL,吸附流度为1.5 mL/min时吸附较强;用0.4 mol/L氯化钠洗脱效果最好,洗脱率可达到82.12%。
Eight kinds of macroporous resins adsorption property had been investigated in isolating and purifying polysaccharide from Cyclocarya paliurus (Batal) Ijinskaja (C. paliurus). The D301R ion exchange resin was selected for purification of polysaccharide. The results showed that the absorption rate of D301R was the highest in eight macroporous resins, elution solvent was 0.4 mol/L NaC1, the recovery rate was 82.12%.
出处
《西北农业学报》
CAS
CSCD
北大核心
2007年第4期275-278,共4页
Acta Agriculturae Boreali-occidentalis Sinica
基金
国家科技部星火计划项目(2002EA730001)
江西省科技厅重点项目(赣科发计字2002163)
关键词
大孔树脂
青钱柳
D301R
多糖
Macroporous resins
C yclocar ya paliurus ( Batal ) Ij inskaj a
D301R
Polysaccharide