滇重楼的抗肿瘤活性成分研究

Anti-tumor constituents from Paris polyphylla var. yunnanensis

目的：研究滇重楼Paris polyphylla Smith var．yunnanensis根茎中具有抗肿瘤作用的活性成分。方法：利用硅胶柱色谱，Sephadex LH-20，反相制备HPLC等手段进行分离纯化，并通过波谱技术进行结构鉴定。采用MTT法对分离到的化合物进行抗肿瘤活性筛选。结果：从醋酸乙酯和正丁醇层中分离得到了6个化合物，鉴定为薯蓣皂苷元-3—O-α-L-呋喃阿拉伯糖基（1→4）-β-D-葡萄糖苷（1）、偏诺皂苷元-3-O-α—L-呋喃阿拉伯糖基（1→4）-β-D-葡萄糖苷（2）、异鼠李素-3—O-β-D-葡萄糖苷（3）、乙基-α—D-呋喃果糖苷（4）、偏诺皂苷元-3—O-α—L-吡喃鼠李糖基（1→4）-[α-L-吡喃鼠李糖基（1→2）]-β-D-葡萄糖苷（5）、偏诺皂苷元-3—O-α—L-吡喃鼠李糖基（1→4）-α-L-吡喃鼠李糖基（1→4）-[α-L-吡喃鼠李糖基（1→2）]-β-D-葡萄糖苷（6）。结论：化合物1—4为首次从滇重楼中分离得到，化合物3和4为首次从重楼属植物中分离得到，化合物5为首次从滇重楼的根茎中分离得到。药理实验表明，化合物1—3，5和6对小鼠肺腺癌细胞LA795都显示出一定的抑制作用，其中化合物5和6最显著。

Objective： To study the anti-tumor active constituents from the rhizome of Paris polyphylla var. yunnanensis. Method： The compounds were isolated by column chromatography with silica gel, and purified by Sephadex LH-20 columu chromafography and reverse-phase preparative, HPLC. The structures were identified by spectroscopic methods. The anti-tumor experiment in vitro, the MTT, was used to screen constituents of P. polyphalla var. yunnanensis. Result： Six compounds were obtained and their structures were identified as diosgenin-3-O-α-L-arabinofuranosyl （ 1 →4 ）-β-D-glycopyranoside （ 1 ）, pennogenin-3-O-α-L-arabino- furanosyl （1→4） -β-D-glycopyranoside （2）, isorhamn etin-3-O-β-D-glycopyranoside （3）, ethyl-α-D-fructofuranoside （4）, pennogenin-3-O-α-L-rhamnopyranosyl （ 1→） - [ α-L-rhamnopyranosyl （ 1→2 ） ] -β-D-glycopyranoside （5） and pennogenin-3-O-α-L-rhamnopyranosyl （ 1→4 ） -α-L-rhamnopyranosyl （ 1→4 ） - [ α-L-rhamnopyranosyl （ 1→2 ） ] -β-D-glycopyranoside （6）. Conclusion： Compounds 1 - 4 were isolated from this plant for the first time, compounds 3 and 4 was firstly isolated from genus Paris, compound 5 was firstly isolated from the rhizome of this plant. The pharmacological results showed that compounds 1-3, 5 and 6 showed certain inhibition, especially, the activities of compound 5 and 6 were the most significant.