摘要
本人用微量全血 EB 病毒转化法建立了杜纳综合征患者的 B 淋巴母细胞株95041。该细胞呈悬浮生长,核型为45,XO,细胞群体倍增时间为62h/代。该细胞株表现为姐妹染色单体交换率(6.27次/细胞)及丝裂霉素 C 诱发姐妹染色单位交换率(13.56次/细胞)微核率(8‰,11.2‰)与正常细胞 JMS95031相比无显著性差异(P>0.05)。
A B—lymphoblastoid cell line(JMS95041)was established by micro—whole blood EB virus transtormation method from a patient with turner sydrome.The results showed that this cell line was a suspension culture with original karyotype 2n=45,XO.The mean cell population doubling time was 62 hours.Neither sister chromatoid exchange(SCE.6.27times/per cell)nor induce sister chormatoid exchange,by MMC(13.56times/per cell)has asignificant difference in contrast with normal control JMS95031.The micro nuclei rate of JMS95041 was 0.8% at normal condition and was 1.12% when treated with MMC showing no significant difference from normal control(P> 0.05).
