基因工程菊粉酶对高浓度蔗糖水解的研究

Study on gene engineering inulinase hydrolyzed hypso-concentration sucrose

对菊粉酶基因工程菌株GS115/INU1进行诱导表达,通过发酵液的分离纯化后,得到在SDS电泳显示单一条带的GS115/INU1酶液。该酶的蔗糖水解酶比活性为1.85U/μg,水解蔗糖最适pH值为4.0,最适温度50℃。加酶量为48U/g底物,对50%蔗糖溶液进行水解,水解时间6h,水解率达到99.7%。对80%蔗糖溶液进行水解,水解时间14h,水解率达到97.8%。

The recombinant inulinase strain GS115/INU1 was induced and expressed. After broth separation and purification, enzyme liquid of GS115/INU1 was obtained which presented single zone in SDS electrophoresis. The optimum temperature and pH of the sucrose hydrolysis were at 50℃ and pH4.0, respectively.Under 48U enzyme per gram sucrose condition, the enzyme hydrolyzed sucrose solution at concentration 50% for 6 hours, and the hydrolysis rate approached 99.7%. The enzyme hydrolyzed sucrose solution at concentration 80% for 14 hours, the hydrolysis rate approached 97.8%.