转染甲状腺hNIS和hTPO基因的神经胶质瘤细胞摄碘功能的研究

Investigation of the radioiodine uptake function in the inferring hNIS and hTPO gene in human glioma cell line in vitro

目的在细胞水平研究转染人钠/碘转运体(hNIS)后的神经胶质瘤细胞的摄碘功能,为最终实现^(131)I治疗非甲状腺肿瘤提供理论依据。方法应用基因转染技术获得稳定表达hNIS的TJ- 905转以及hNIS和人甲状腺过氧化物酶(hTPO)瞬时共转染的TJ-905共,然后研究两种细胞的摄碘功能以及^(131)I的细胞毒作用。结果TJ-905转摄^(125)I活性增高(45.99±0.19)倍,其有效T_(1/2)约为5 min;TJ-905共的^(125)I有机化程度增高,有效T_(1/2)延长至10 min。同时TJ-905转的细胞增殖率降低,第7天降至(40.28±0.37)%,其细胞克隆存活率亦降至(31.83±0.52)%。结论转染hNIS基因的神经胶质瘤细胞具有较强的摄碘功能,并且^(131)I对其具有较强的抑制增殖作用。

Objective To provide an objective evidence for radioiodine therapy in nonthyroid tumor, we investigated the glioma cell＇s radioiodide uptake function. Methods Based on the cloning of human sodium/iodide（hNIS）, stably expressing hNIS human glioma cell lines TJ-905-t and transient co-transfection of hNIS and hT-PO cell lines TJ-905-co were obtained, their radioactive 125I uptake function and ^131I inhibitory effect on cell proliferation were investigated. Results The uptake of ^125I was （45.99 ±0.19） times higher in TJ-905-t group than that in control group, its efficient half life was about 5 minutes. The amount of organified 125I present in TJ-905-co group was 4 times higher than that in TJ-905-t group. Furthermore, its efficient half life prolonged to 10 minutes. Moreover, the proliferative activity in TJ-905-t group declined gradually. It was （40.28± 0.37） % on the seventh day after seed, accordingly its colony formation number strikingly reduced to（31.83± 0.52）%. Conclusions After the transfection of hNIS gene, the uptake of ^125I was markedly higher in glioma cell lines, furthermore the inhibitory effect of^ 131I on cell proliferation was enhanced in TJ-905-t.