早期液体复苏对严重烧伤大鼠下腔静脉血TC、TG、HDL、ALP含量的影响

The Effect of the Immediately Fluid Resuscitation on TC,TG,HDL,ALP Levels of the Inferior Vena Cava Blood

目的研究早期液体复苏对严重烧伤大鼠下腔静脉血中TC(总胆固醇)、TG(三酰甘油)、HDL(高密度酯蛋白)、ALP(碱性磷酸酶)含量的影响。方法选择健康成年SD大鼠144只,体重(250±20)g,随机分为A、B、C、D 4组,每组为36只。A组:正常对照组;B组:单纯烫伤组;C组:早期复苏组;D组:延迟复苏组。A组SD大鼠麻醉后背部给予30%面积的模拟烫伤;B、C、D 3组SD大鼠麻醉后背部给予30%TBSAⅢ度烫伤,伤后不补液;C组烫伤后立即按parkland公式采用复方乳酸钠林格氏液进行液体复苏;D组烫伤后6 h给予液体复苏,方法同C组。各组大鼠分别于伤后12 h、24 h、48 h、72 h7、d、21 d采下腔静脉血(每时相点6只大鼠),全自动生化分析仪检测血TC、TG、HDL、ALP的含量。结果严重烧伤后下腔静脉血TC、TG、ALP的含量均上升,正常对照组、早期复苏组、延迟复苏组、单纯烫伤组各时相点TC、TG、ALP的含量均依次增高,与其余3组相比,有显著性差异(P<0.05,P<0.01);HDL的含量则随正常对照组、早期复苏组、延迟复苏组、单纯烫伤组依次降低,与其余3组动物相比,早期复苏组也有显著性差异(P<0.05,P<0.01)。结论早期液体复苏可以减轻肝脏脂肪变性,可较好维持下腔静脉血中TC、TG、HDL、ALP含量的相对稳定。

Objective To explore the effect of the immediately Fluid Resuscitation on TC, TG, HI）L, ALP levels of the inferior vena cava blood. Methods One hundred and forty four Sprague- Dawley rats ,weighing 230-270 g, were randomly divided into four groups： normal control（A, NC） ,only burn（B, SB）, immediately fluid resuscitation（C, IFR）and delayed fluid resuscitation （D,DFR）, with 36 animals in each group. Each rat was anesthetized with ketamine. NCG S-D rats were subjected to 30% sham scald burns, other three groups were subjected to 30% full-thickness scald burns. IFRG received [4 mL/（%TBSA · kg）] of Ringe solution immediately after burn injury. DFRG received Ringer＇solution 6 h postburn as IFRG. SBG didn＇t receive resuscitation. Results Severe burn resulted in elevation of TC, TG, ALP, but reduction of HDL. The levels of TC, TG, and ALP in NCG, IFRG, DFRG, and SBG increased. At the same time, the level of HDL reversed. Conclusion Fluid resuscitation immediately after burn can ameliorate hepatic fatty degeneration and stabilize the levels of TC,TG,HDL and ALP of the inferior vena cava blood.