骨康合剂对兔实验性膝骨关节炎关节软骨中iNOS表达的影响

Effect of GuKang Mixture on iNOS Experssion in Articular Cartilage of Knee Osteoarthritic in Rabbits Model

目的采用免疫组化及图象分析方法检测骨康合剂对兔实验性骨关节炎关节软骨中诱导型一氧化氮合成酶(iNOS)的表达的影响,以探讨其防治膝骨关节炎的作用机理。方法新西兰兔32只,建立兔Hulth膝骨关节炎模型,术后随机分为3组:模型组、关节壮骨丸组和骨康合剂组。个组采取相应处理方法,分别于术后11周、14周处死取材,进行免疫组化并采用图象分析系统对其表达检测。结果3组iNOS的表达强度相比较,骨康合剂组明显低于关节壮骨丸组和模型组。结论iNOS在膝骨关节炎的病理进程中具有重要作用,骨康合剂可通过下调其在关节软骨中的表达,达到防治膝骨关节炎的作用。

Objective To investigate the influence of iNOS expression in articular cartilage of the rabbit knee osteoarthritic model with gukang mixture treatment by Using the immunohistoche mistry and image analysis methods and explore the mechanism of treating knee osteoarthritis with gukang mixture. Methods Thirty-two New Zealand rabbits were divided into three groups：gukang mixture group, Zhuangguguanjie Wan group and untreated group postoperation. All rabbits were established the Hulth knee osteoarthritic model. The three groups were dealt with different therapy respectively and taken samples at postoperation 11 and 14 weeks by killing 4 rabbits in each group. All samples were undertaken immunohistochemistry and image analysis methods in order to test their iNOS expressing intensity. Results The size of iNOS positive expressing intensity of gukang mixture group was notable lower than those of Zhuangguguanjie Wan group and the untreated group. Conclusion iNOS takes a key role in the osteoarthritic pathological process and Gukang mixture could prevent and treat the knee osteoarthritis by down-regulating the expression of iNOS.