摘要
目的建立HPLC法测定发酵液中苏氨酸含量的方法。方法采用OPA试剂进行柱前衍生，用氨基柱，以流动相A：PH值7．80的磷酸二氢钠溶液。流动相B：乙腈-甲醇-水为45：45：10，进行梯度洗脱，柱温40℃，检测波长：338nm，流速：2mL·min^-1，程序进样。结论浓度在0．058mg·mL^-1～1．167mg·mL^-1范围内呈良好线性（r=0.9999），重现性好，快速、简便、准确。
OBJECTIVE To set up an HPLC method to analysis the content of Threonine in fermental solution. METHODS Pre-column derivation was carried out by OPA reagent. The NH2 column was adopted, mobile phase A：sodium dihydrogen phosphate solution （pH = 7.80）. mobile phase B： Acetonitrile- methanol- water = 45 ： 45 ： 10 column temperature was 40℃ ,detective wavelength：338nm, flow rate：2mL·mL^-1. CONCLUSION The calbration curve were linear in the range of 0. 058mg·mL^-1 -1. 167mg·mL^-1（ r =0. 9999）. The method was repeatable,rapid,simple and accurately.
出处
《齐鲁药事》
2007年第7期406-407,共2页
qilu pharmaceutical affairs
