薄层扫描法检测血清、尿中磺胺甲唑和甲氧苄氨嘧啶

Identification of sulfamethoxazole and trimethoprim in serum and urine by TLC

本文建立了血清、尿中ＳＭＺ和ＴＭＰ的薄层扫描定性定量检测方法。血清和尿中ＳＭＺ的线性检测范围为４～１２８μｇ／ｍｌ，最小检出浓度为１．６μｇ／ｍｌ；ＴＭＰ的线性检测范围为１～３２μｇ／ｍｌ，最小检出浓度为０．８μｇ／ｍｌ。

A rapid TLC qualitative and quantitative analysis of sulfamethoxanole (SMZ) and trimethoprim (TMP) in human serum and urine was described.Carbamazepine was used as the internal standard.Ethylacetate was used as the extraction reagent.The extraction curves of SMZ in serum and urine were linear over the range of 4 to 128ug/ml.The limit of detection was 1.6 μg/ml.The extratin calibration curves of TMP in serum and urine were linear over the range of 1 to 32μg/ml.The limit of detection was 0.8 μg/ml.This method has been applied to identify SMZ and TMP in serum and urine of two overdosed cases.