β-氨基丁酸诱导辣椒细胞色素P450基因的克隆及其序列分析

Molecular Cloning and Sequence Analysis of Cytochrome P450 in Pepper (Capsicum annuum L.) Induced by β-aminobutyric Acid (BABA)

用RT-PCR和RACE方法,从β-氨基丁酸(β-aminobutyric acid,BABA)诱导辣椒叶片的基因表达体系中克隆出CYP92A的全长cDNA序列。在BABA处理后,CYP92A基因以一种快速而短暂的方式作出应答。生物信息学分析表明,该基因编码一条509个氨基酸残基的多肽,含有一段细胞色素P450保守的血红素结合区域,由此CYP92A被确定是细胞色素P450。通过系统进化分析,CYP92A属于多基因家族P450的92A亚家族。结合已报道92A亚家族成员的研究,推测CYP92A参与植物的防御反应。通过生物信息学分析蛋白结构,对CYP92A催化反应特性进行了讨论。为进一步探讨BABA的诱导机理奠定了基础。

The complete CYP92A was amplified by RT-PCR and RACE, which expressed in pepper （ Capsicum annuum L. ） leaf was rapidly and transiently induced by β-aminobutyric acid （BABA）. The analysis of bioinformatics showed that CYP92A gene encoded a 509-amino acid protein which contained a conserve hemebinding region of cytochrome P450. Therefore, it was confirmed that CYP92A is cytochrome P450. By phylogenetic analysis, it was belived to belong to 92A sub-family of P450. According to researches of other 92A subfamily genes reported, it is speculated conferred that CYP92A may play a role in plant defence response. Based on bioinformatics analysis of protein struction, the property of CYP92A in catalytic reaction was also discussed in this paper. This study had significance for research on indued-resistance mechanisms of BABA.