极早熟油桃幼胚离体培养成苗相关因素探讨

Studies on the Correlative Factors for Normal Seedling Development in Immature Embryo Culture of Ultra-early Maturing Nectarine

以极早熟油桃‘超红短枝’的幼胚为试材,在离体培养条件下,研究了不同胚龄、低温处理时间、蔗糖浓度、6-BA、GA3浓度配比及种皮等因素对幼胚发育和成苗的影响。结果表明:果实发育56d时,平均PF值仅为0·086。在各处理中,以低温处理80d、糖浓度6%对幼胚的发育和成苗效果最佳。6-BA、GA3配比对经80d低温处理的幼胚影响较小,9mg/L的6-BA对30d低温处理的幼胚发育有一定的促进作用。在低温条件下,带种皮培养对幼胚PF值增大和提高成苗率的效果明显好于不带种皮培养的。

The effects of different embryo developmental stages, treatment time of low temperature, 6-BA and GA3 levels, sucrose concentration in culture medium and seed coats on immature embryo development and normal seedling rate were studied with ultra-early maturing nectarine variety Super Red Spur. The results indicated that the average PF value was only 0. 086 when fruits developed up to 56 days. Among the all treatments, the treatment with low temperature for 80 days and 6% sucrose concentrations in the culture medium had the highest effects on immature embryo growth and normal seedlings rate. Moreover, different 6-BA and GA3 concentrations showed little influence on the immature embryos cultured for 80 days in low temperature; but 9 mg/L 6-BA had some promotion on the immature embryos development for 30 days. The PF value and the percent of normal seedling of immature embryos with seed coats were found better compared with the embryo without seed coats when cultured at low temperature condition.