摘要
目的：探讨新的凋亡抑制基因survivin在膀胱移行细胞癌（bladder transitional cell cancer，BTCC）组织中的表达，及其与bcl-2基因表达的相关性。方法：应用免疫组织化学链霉抗生物素蛋白-过氧化物酶连接法（SP法），检测survivin和bcl-2在58例BTCC组织中的表达．另选10例癌旁正常组织作对照。结果：survivin基因在癌旁正常组织中无表达，而在BTCC中呈高表达，survivin的表达与BTCC的临床分期和病理分级密切相关。58例BTCC组织中，41例表达阳性，占70．7％。同时发现survivin的表达与bcl-2的表达也密切相关。结论：survivin基因的异常表达而引起的细胞凋亡抑制，在BTCC的发生发展中起一定作用，其过度表达提示BTCC预后不良。survivin的表达与BTCC组织中的bcl-2的异常表达密切相关。
Objective：To study the expression of a novel inhibitor gene of apoptosis, survivin, in bladder transitional cell cancer, and its relationship with the expression of bcl-2 genes. Methods：The expression of survivin and bcl-2 proteins was detected in 10 normal bladder tissues and 58 bladder transitional cell cancer tissues with striptavidin-biofin pemxidase（SP）methhod. Results： Survivin was expressed in 41 of 58 （70,7%） cases of BTCC. In contrast, normal bladder epithelium did not express survivin. Overexpression of survivin was related to the tumor grade and clinical stage.The expression of survivin were closely correlated with bcl-2 expression. Conclusion ：Apoptosis is inhibited by survivin alone or in cooperation with bcl-2, which may participate in the onset and progression of bladder transitional cell cancer. Survivin could be a new diagnostic/therapeutic target in bladder trandifional cell cancer.
