铝对体外培养大鼠神经细胞凋亡的研究

Study of neurocytes apoptosis in vitro culture rat induced by aluminum

目的研究铝对体外培养大鼠神经细胞凋亡的作用。方法体外培养0-3天大鼠单纯神经元、神经胶质细胞及混合培养神经细胞,并用不同浓度AlCl3.6H2O处理,分为对照组、低、中、高剂量组（分别为0、0.5、1.0和2.0mmol/L）,做光镜观察、荧光染色及流式细胞检测。结果①在光镜下和荧光染色法观察,可以见到神经元有凋亡细胞的典型形态学改变,但在神经胶质细胞及混合培养细胞中没有观察到凋亡的典型形态学改变。②神经元的流式检测结果显示,铝可以诱导神经元凋亡,且其早期、晚期及总凋亡率与铝的作用剂量有关;而神经胶质细胞的流式检测结果在染毒剂量组中未发现凋亡率的显著升高,其凋亡率在各组间差异均无显著性;混合培养神经细胞的流式检测结果在染毒剂量组中亦有凋亡率的显著升高,但其凋亡率升高的程度远低于神经元。结论低浓度铝可诱导神经元细胞凋亡。

Objective To study the rat neurocytes apoptosis induced by aluminum. Methods 0 - 3 day rats neurons, neuroglia cells and co-cultured neurocytes were cultured, which were treated by different concentration of AlCl3 · 6H2O, the cells by light microscope, fluorescent staining and fluorescent analysis were observed. Results （ 1 ） Under light microscope and fluorescent staining, Al could cause strong typical morphology apoptosis changes in neurons, which appeared more earlier and typical than those in neuroglia cells and co-cultured neurocytes. （2）The results of fluorescent analysis reviewed that the early stage, late stage and total apoptosis rate of neurons apoptosis could be associated with Al concentration. However, Al could not induce neuroglia cells and co-cultured neurocytes apoptosis at the low and mid doses of aluminum, the apoptosis rates in four groups demonstrated no significance, in contrast only significant difference between control group and high dose group. Conclusion It was suggested that Al could induce apoptosis of neurocytes in rat.