健脾补肾、和解泄浊法抑制肾间质纤维化的实验研究

Effects of Reinforcing Kidney and Exhausting Toxic Mixture on Renal Interstitial Fibrosis in Rats

目的观察补肾排毒合剂对单侧输尿管梗阻(UUO)后大鼠肾间质α-平滑肌肌动蛋白(α-SMA)、Ⅳ型胶原(ColⅣ)表达的影响及对肾间质纤维化的保护作用。方法采用UUO诱导肾间质纤维化的动物模型,将48只Wistar大鼠随机分为假手术组(Sham)、模型组(UUO)和补肾排毒合剂治疗组(REM),每组分别于造模后3、10、20、30d各处死4只,取左肾组织行HE染色,动态观察肾脏病理学变化,评定各组肾小管间质损害程度,以免疫组化方法测定肾组织中α-SMA、ColⅣ。结果与UUO组比较,REM组大鼠肾间质病变程度明显减轻,α-SMA、ColⅣ在肾间质中的表达也有明显降低。结论补肾排毒合剂可通过降低α-SMA、ColⅣ的表达,减少肾间质细胞外基质合成,延缓肾间质纤维化的进展。

Obujective： To explore the preventive and theraputic effects of Reinforcing Kidney and Exhausting Toxic Mixture（REM） on renal interstitial fibrosis by unilateral ureteral obstruction. Methods： Rar unilateral ureteral ohstruction（UUO） model was used for the study. 48 Wistar rats were randomly divided into 3 Groups of 16 rats： Shan - oparation, UUO and UUO ＋ REM. For rats from each groups were sacrificed at 3, 10, 20, 30days after U- UO. left kidney of each rats was stained with hematoxylind eosin method. Evaluate the degree of tubula - interstitial damage. α- smooth muscle action（α- SMA） and collagen IV were identified using an imunohistochemistry tech- nique. Reults： The real pathologic lesions were significantly alleviated in REM treated rats as compared with those inflicted with UUO. Immunohistochemisry staining studies also showed a marked decrease in the expression of α - SMA and collagen IV in. Conclusions： REM cold renard the expression of α - SMA and collagen IV in renaltissues, which may decrease matrix secretion and retard progression of renal interstitial fibrosis.