磷酸化Stat3和磷酸化Akt及磷酸化ERK1/2在表皮良恶性肿瘤中的表达

Expression of phosphorylated-Stat 3,phosphorylated-Akt and phosphorylated-ERK1/2 in benign and malignant epidermal tumors

目的探讨磷酸化细胞信号传导与转录活化因子3(p-Stat3)和磷酸化Akt(p-Akt)及磷酸化细胞外信号调节激酶(p-ERK1/2)在表皮良恶性肿瘤中的表达。方法采用免疫组化的方法检测脂溢性角化病,光线性角化病,鲍恩样丘疹病,基底细胞上皮瘤,鲍恩病,鳞状细胞癌中p-Stat3,p-Akt及p- ERK1/2的表达。结果p-Stat3,p-Akt及p-ERK1/2阳性表达率在表皮恶性肿瘤中明显高于良性肿瘤(P均<0.05)。p-Stat3和p-Akt的阳性表达率在表皮恶性肿瘤中从高到低依次为鳞状细胞癌>鲍恩病>基底细胞上皮瘤,而p-ERK1/2的阳性表达率无差异(P>0.05)。在表皮恶性肿瘤中,p-Stat3和p-Akt的阳性表达呈显著正相关(P<0.05).而p-ERK1/2的阳性表达与p-Star3、p-Akt的阳性表达之间无相关性(P>0.05)。结论p-Stat3,p-AKT和p-ERK1/2参与表皮恶性肿瘤的发病。

Objective To investigate the expression ofp-Stat3, p-Akt and p-ERK1/2 in benign and malignant epidermal tumors. Methods Immunohistochemistry SP method was used to detect the expression ofp-Stat3, p-Akt and p-ERK1/2 in specimens from 20 cases of seborrheic keratosis （ SK ）, 20 cases of actinic kcratosis （ AK ）, 20 cases of bowenoid papulosis （ BP ）, 20 cases of basal cell carcinoma （ BCC ）, 20 cases of Bowen＇s disease （BD） and 50 cases of squamous cell carcinoma （SCC）. Results The expression rates of p-Stat3 ,p-Akt and p-ERK1/2 were significantly higher in malignant epidermal tumors than those in benign epidermal tumors （ all P 〈 0.05 ）. In malignant tumors, the expression rates of p-Stat3 and p-Akt were in decreasing order in SCC, BD and BCC, while the expression of p-ERK1/2 was of no significant difference （ P 〉 0.05 ）. There was a positive correlation between the expression of p-Stat3 and p-Akt （ P 〈 0.05 ）, but the expression of p-ERK1/2 did not correlate with that of p-Stat3 and p-Akt （ both P 〉 0.05）. Conclusion p-Stat3, p-Akt and p-ERK1/2 may take part in the pathogenesis of malignant epidermal tumors.