人参胡核汤对人前列腺癌PC-3细胞增殖的抑制作用

The inhibiting effects of Renshen Huhe Tang on PC-3 human prostate cancer cell proliferation

目的探讨人参胡核汤对人前列腺癌PC-3细胞的增殖抑制作用。方法通过血清药理学的方法,制备人参胡核汤高、中、低剂量及空白组的血清。各组与PC-3细胞作用48h、72h后,光镜观察细胞形态学改变;MTT法观察细胞增殖的抑制率;RT-PCR技术检测前列腺特异性抗原(PSA)表达。结果人参胡核汤含药血清各组作用48h、72h后,表现出不同程度的细胞皱缩,边缘毛糙,细胞核固缩或碎裂,细胞体积缩小;各组均可抑制PC-3细胞增殖;降低PSA表达水平。上述指标中,以人参胡核汤含药血清高剂量组作用48h的效果最为明显。结论人参胡核汤含药血清可使PC-3细胞的形态发生改变、抑制PC-3细胞增殖、降低PSA的表达。

Objective To observe the inhibiting effects of Renshen Huhe Tang on PC-3 human prostate cancer cell proliferation. Methods With the experimental method of ＂serological pharmacology＂, SD rats were divided into blank serum group, treated group with high dosage, middle dosage and low dosage serum Renshen Huhe Tang. After 48h and 72h, cell morphology was seen to change; MTT assay was used to determine the inhibiting effects of PC-3 cell proliferation; RT-PCR method was used to study the regulative effect of Renshen Huhe Tang on prostate marker genes of prostate specific antigen. Results Through the different groups, the changes as chromatin condensation and nuclear fragmentation, the cells shrunken; the cell growth inhibition, the proliferation speed decrease; deduction of the expression of prostate specific antigen could be seen. Among the indexes, the most evident changes were seen in high dosage serum Renshen Huhe Tang of 48 hours. Conclusion Renshen Huhe Tang can change PC-3 cell morphology, restrain PC-3 cell proliferation and depress prostate specific antigen to exert the effects of repelling tumor.